Precautions for using the autoclaves / steam sterilizers

Take out the inner bucket, and then add an appropriate amount of water to the outer pot until the water surface is level with the triangular shelf. I wonder if you have noticed this when filling the water. It should be noted that the mouth ends of the Erlenmeyer flask and the test tube should not be in contact with the wall of the barrel. This is because we do not want the condensed water to wet the paper wrapping the mouth and penetrate into the cotton plug.

Another point is that capping is a common operation when we operate steamers, but during the operation, we often make mistakes. We must insert the exhaust hose on the cover into the exhaust groove of the inner barrel. . Then tighten the two opposite bolts at the same time in a symmetrical manner to prevent air leakage.

After the required time is completed, be sure to cut off the power supply or turn off the gas to allow the temperature inside the steamer to drop naturally. At this time, when the pressure on the pressure gauge drops to 0, open the exhaust valve, loosen the bolts, open the cover, and carefully take it out. thing. Of course, if you find that the pressure gauge has not dropped to 0, we must not open the exhaust valve, because as long as the exhaust valve is opened, the pressure in the pot will suddenly drop, causing the culture medium in the container to rush out due to the imbalance of internal and external pressure. The mouth of the flask will cause the cotton plug to contaminate the culture medium, and our operation will end in failure.